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For research use only

Research tool

Reconstitution calculator

Enter the vial's milligrams and the bacteriostatic water added to get the resulting concentration. A laboratory measurement reference.

Lyophilized material in vial
Bacteriostatic water added 2 mL

This vial at each volume

  • 1 mL10.0 mg/mL
  • 2 mL5.00 mg/mL
  • 3 mL3.33 mg/mL
  • 5 mL2.00 mg/mL

Resulting concentration

5.00mg/mL

5,000 mcg per mL

50.0 mcg
per 1 unit (0.01 mL)
500 mcg
per 10 units (0.1 mL)

Amount by draw volume, U-100 graduated syringe

Syringe unitsVolumeMaterial drawn
5 units0.05 mL250 mcg
10 units0.10 mL500 mcg
20 units0.20 mL1,000 mcg
25 units0.25 mL1,250 mcg
50 units0.50 mL2,500 mcg

One graduation on a U-100 barrel is 0.01 mL. A measurement reference, not guidance on quantity or use.

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What the number means

Reconstitution is dissolving a lyophilized (freeze-dried) solid back into a liquid. The mass in the vial does not change when the diluent goes in, so the concentration is that mass divided by the volume added. A 10 mg vial taken up in 2 mL is 5 mg/mL; the same vial in 5 mL is 2 mg/mL.

Micrograms and milligrams are the same measurement at different scales: 1 mg is 1,000 mcg. Both are shown because certificates of analysis and specification tables do not consistently use one or the other.

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